Studies on Immunological Assay of Human Chorionic Gonadotropin. I. Purification and Immunological and Physicochemical Properties of Human Chorionic Gonadotropin
نویسندگان
چکیده
منابع مشابه
Immunological studies with a commercial preparation of human chorionic gonadotropin.
Immunological methods are being used to determine human chorionic gonadotropin (HCG) in urine and serum during pregnancy (1-4) and also to localize it in tissues and tumors (5). The antisera used in these studies (1-5) were produced by immunizing rabbits with HCG preparations of widely varying specific activities. The HCG-HCG antisera characterization studies that were performed primarily with ...
متن کاملHuman Chorionic Gonadotropin
A highly active preparation of human chorionic gonadotropin has been obtained by simple methods of column chromatography on diethylaminoethyl Sephadex and Sephadex G-100. The preparation appeared homogeneous by disc and immunoelectrophoresis, ultracentrifugation, and end group analysis. It assayed at 12,000 i.u. per mg by the rat prostrate gland and accessory sex organ method. The ~20,~ value w...
متن کاملBiological properties of human chorionic gonadotropin.
The present study is designed to elucidate certain biological characteristics of urinary chorionic gonadotropin and to see what differences could be demonstrated between HCG in normal pregnancy and in the trophoblastic diseases. We have attempted the biological characterization of HCG using crude HCG and the purified HCG (12 ,000 I.U./mg) by zone electrophoresis on starch grain and chromatograp...
متن کاملPhysicochemical characterization of native and asialo human chorionic gonadotropin.
Human chorionic gonadotropin (HCG) was desialized by treatment with neuraminidase to yield asialo-HCG, and comparative physicochemical studies were carried out on HCG and asialo-HCG. The E:trn value at 276 nm was 5.47 for HCG and 5.72 for asialo-HCG. Intrinsic viscosities of HCG and asialo-HCG at near isoionic point were estimated to be 3.4 and 3.2 ml per g, respectively. HCG had an isoionic po...
متن کاملQuantification of Human Chorionic Gonadotropin by Bovine Serum Albumin Nanoparticles
Background and Aims: Some nanoparticles can be used in immunoassays to increase sensitivity. This study aimed to evaluate a novel nano-immunoassay based on bovine serum albumin nanoparticles (BSA NPs). Materials and methods: At first, the nanostructure was synthesized, and then applied as a tag in the nano-immunoassay. Then the concentration of β-subunit of human chorionic gonadotropin ...
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ژورنال
عنوان ژورنال: YAKUGAKU ZASSHI
سال: 1978
ISSN: 0031-6903,1347-5231
DOI: 10.1248/yakushi1947.98.3_369